Lab Assistant 1 - Part Time - 69726-1A

The Department of Anatomy and Neurobiology is a world-leading neuroscience department where molecular, anatomical, and physiological discoveries are seamlessly integrated into developing new treatments for neurological diseases. This research position will be funded by PI’s current extramural awards (NIH, CIRM, or Department of Defense). This position represents a unique and exciting opportunity to participate in state-of-the-art research to elucidate the mechanistic basis of cancer and cancer therapies on brain function and mitigate these neurodegenerative consequences using pharmacological, genetic, or regenerative approaches. The Lab Asst-I is expected to execute several specialized skills in Neuroscience research. 1. In consultation with PI and senior lab members, conduct step-by-step optimization of animal behavior protocol 2. In consultation with PI and senior lab members, optimize and conduct automated tracking, storage, and monitoring of animal behavior videos using specialized scientific modules (Noldus Ethovision, Anymaze, FreezeFrame, etc.) 3. Optimize and conduct dual/triple immunofluorescence staining, laser scanning confocal microscopy and 3D algorithm-based volumetric quantification of confocal z stacks to acquire and analyze the cellular and synaptic marker data. 4. Intra-cardiac perfusion of animal tissues, cryo-preservation, and sectioning using cryo-stat. 5. Collection and analysis of animal behavior, confocal microscopy, cell culture, and biochemical data sets, and collate/organize data sets for statistical analysis. 6. Laboratory maintenance, including working and standard solution preparation, conducting specialized molecular and cellular analytical techniques, including cell culturing, ELISA, Western Blotting, PRC/RT-PCR, etc. 7. Small animal handling, dosing, animal care, major and minor survival surgeries, including peripheral organ and intracranial surgeries. 8. Small animal irradiation using specialized irradiation equipment. 9. Optimize and conduct BLI and CT scanning techniques for in vivo tracking of tumors. 10. Optimize the performance of computers used for data analysis, animal behavior, confocal microscopy, etc. 11. Train and supervise undergraduate and graduate students to perform animal cognitive function studies. 12. Train and supervise undergraduate and graduate students to perform cellular and molecular studies using animal and human tissues. 13. Communicate student’s data and design student’s research projects with the PI. 14. Collect and prepare data sets for presentation at scientific conferences. 15. Collect and prepare data sets for scientific publications. Required: Prior experience in basic laboratory skills, laboratory and chemical hygiene, knowledge of MS Office, and data analysis computer modules. Desirable: Prior experience in brain immunofluorescence staining, confocal microscopy, in silico analysis of immunoreactivity.